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Amplification of human genomic sequences by human papillomaviruses universal consensus primers

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APA

Fernández Contreras, María Encarnación & Sarriá, C. & Nieto, S. & Lazo, P.A. (2000 ) .Amplification of human genomic sequences by human papillomaviruses universal consensus primers.

ISO 690

Fernández Contreras, María Encarnación & Sarriá, C. & Nieto, S. & Lazo, P.A.. 2000 .Amplification of human genomic sequences by human papillomaviruses universal consensus primers.

https://hdl.handle.net/20.500.12080/45069
dc.contributor.author Fernández Contreras, María Encarnación
dc.contributor.author Sarriá, C.
dc.contributor.author Nieto, S.
dc.contributor.author Lazo, P.A.
dc.date.accessioned 2025-01-09T18:23:43Z
dc.date.available 2025-01-09T18:23:43Z
dc.date.created 2000
dc.date.issued 2000
dc.identifier.uri https://hdl.handle.net/20.500.12080/45069
dc.description.abstract Detection of human papillomaviruses DNA (HPV) in tumour samples is often determined by a PCR based approach with standard universal consensus oligonucleotides. It is shown that these primers under the same ampli®cation conditions can amplify a human genomic sequence of 1361 nucleotides in oral carcinomas and normal DNA samples. This sequence is detected more easily as the copy number of HPV DNA decreases. Therefore, in tumour samples that have a low copy number of HPV or that are contaminated by normal tissue there is a potential risk of misidenti®cation of the presence of HPV if this observation is not taken into account. © 2000 Elsevier Science B.V. All rights reserved. Keywords: HPV; Cancer; Oral cancer; Cervical cancer; Tumour; Virus; PCR es_ES
dc.format application/pdf es_ES
dc.language eng es_ES
dc.relation.ispartof Journal of Virological es_ES
dc.rights CC-BY es_ES
dc.rights.uri http://creativecommons.org/licenses/by/4.0/deed.es es_ES
dc.title Amplification of human genomic sequences by human papillomaviruses universal consensus primers es_ES
dc.type info:eu-repo/semantics/article es_ES
dc.rights.accessrights info:eu-repo/semantics/restrictedAccess es_ES
dc.identifier.location N/A es_ES


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