Amplification of human genomic sequences by human papillomaviruses universal consensus primers

Abstract

Detection of human papillomaviruses DNA (HPV) in tumour samples is often determined by a PCR based approach with standard universal consensus oligonucleotides. It is shown that these primers under the same ampli®cation conditions can amplify a human genomic sequence of 1361 nucleotides in oral carcinomas and normal DNA samples. This sequence is detected more easily as the copy number of HPV DNA decreases. Therefore, in tumour samples that have a low copy number of HPV or that are contaminated by normal tissue there is a potential risk of misidenti®cation of the presence of HPV if this observation is not taken into account. © 2000 Elsevier Science B.V. All rights reserved. Keywords: HPV; Cancer; Oral cancer; Cervical cancer; Tumour; Virus; PCR