APA
Díaz López, Emilia Elena & Ríos Díaz, Arturo & Vanegas Calderón, Oriana & Lajkó, Eszter & Ponte-Sucre, Alicia & Kohidai, László (2021 ) .Chemotaxis in Leishmania (Viannia) braziliensis: Evaluation by the two-chamber capillary assay.
ISO 690
Díaz López, Emilia Elena & Ríos Díaz, Arturo & Vanegas Calderón, Oriana & Lajkó, Eszter & Ponte-Sucre, Alicia & Kohidai, László. 2021 .Chemotaxis in Leishmania (Viannia) braziliensis: Evaluation by the two-chamber capillary assay.
https://hdl.handle.net/20.500.12080/39379
Resumen:
Chemotactic responses play a significant role during Leishmania (V.) braziliensis differentiation through its life
cycle and during infection. The aim of this description has been to portray the modified ¿two-chamber capillary
chemotaxis assay¿ as a technique useful for quantitative in vitro evaluation of Leishmania chemotaxis after
reviewing the methods described until now to assess chemotaxis in vitro in Leishmania sp. This valued simple
and reproducible method convenient for parasite migration determination, was tested by the use of controlled
changes in monosaccharide (D-glucose and D-fructose) concentrations as referent ligands. The validation of
the method demonstrates that this technique is useful to evaluate the relationship existing between parasite
migration towards the monosaccharides and sugar concentration. This means that within specific ranges, parasites
attracted by the monosaccharide migrate towards more concentrated solutions and accumulate (higher number
of parasites) at that spot. Interestingly, both the time course of the experiment and the osmolality of the solution
influence parasite migration capacity. Our validation suggests that this improved methodology quantitatively
evaluates taxis of Leishmania towards/against different substances. On the basis of our herein presented data, we
conclude that this technique is a novel, rapid and reliable screening method to evaluate chemotaxis in Leishmania.