APA
Delgado Bonet, Pablo & Tomeo Martín, Beatriz Davinia & Ortiz Díez, Gustavo & Perisé Barrios, Ana Judith .Tumor-Homing of Mesenchymal Stem Cells Infected with Oncolytic Virus in a Canine Patient.
ISO 690
Delgado Bonet, Pablo & Tomeo Martín, Beatriz Davinia & Ortiz Díez, Gustavo & Perisé Barrios, Ana Judith. Tumor-Homing of Mesenchymal Stem Cells Infected with Oncolytic Virus in a Canine Patient.
https://hdl.handle.net/20.500.12080/32719
Résumé:
Abstract: Intravenous administration of oncolytic adenovirus (OAds) can be challenging, although
various vehicles for the delivery of the virus to the tumor have been described. The efficacy of
mesenchymal stem cells (MSCs) as a virus vehicle has been reported in mouse models and canine
and human patients, but the actual action mechanism has never been described in patients. It is
of importance to determine whether MSCs infected with OAds can reach the tumor and release
the virus in a clinical setting. For this purpose, GFP-labeled MSCs were infected with an OAd and
inoculated into a companion dog diagnosed with spontaneous lung carcinoma. Forty-eight hours
later, the tumor was excised and analyzed microscopically by flow cytometry for GFP fluorescence
detection, and a cellular culture was established. Peripheral blood samples were taken to quantify
the oncolytic adenovirus by qRT-PCR. Green fluorescence cells detected in the cellular culture by
microscopy and flow cytometry revealed 0.69% GFP-positive cells in the tumor. OAd in peripheral
blood was confirmed by qRT-PCR during follow-up. For the first time, the tumoral-homing capacity
of OAds infected-MSC has been confirmed in a clinical setting, helping to explain the clinical response
mechanism, whose efficacy was previously reported in canine and human patients.
Keywords: Celyvir; oncolytic virus; tumor-homing; virotherapy