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Selective miRNA Modulation Fails to Activate HIV Replication in In Vitro Latency Models

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López Huertas, María Rosa & Morín, Matías & Madrid Elena, Nadia & Gutiérrez, Carolina & Jiménez Tormo, Laura & Santoyo, Javier & Sanz Rodríguez, Francisco & Moreno Pelayo, Miguel Ángel & García Bermejo, Laura & Moreno, Santiago (2019-09 ) .Selective miRNA Modulation Fails to Activate HIV Replication in In Vitro Latency Models.

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López Huertas, María Rosa & Morín, Matías & Madrid Elena, Nadia & Gutiérrez, Carolina & Jiménez Tormo, Laura & Santoyo, Javier & Sanz Rodríguez, Francisco & Moreno Pelayo, Miguel Ángel & García Bermejo, Laura & Moreno, Santiago. 2019-09 .Selective miRNA Modulation Fails to Activate HIV Replication in In Vitro Latency Models.

https://hdl.handle.net/20.500.12080/39726
dc.contributor.author López Huertas, María Rosa
dc.contributor.author Morín, Matías
dc.contributor.author Madrid Elena, Nadia
dc.contributor.author Gutiérrez, Carolina
dc.contributor.author Jiménez Tormo, Laura
dc.contributor.author Santoyo, Javier
dc.contributor.author Sanz Rodríguez, Francisco
dc.contributor.author Moreno Pelayo, Miguel Ángel
dc.contributor.author García Bermejo, Laura
dc.contributor.author Moreno, Santiago
dc.date.accessioned 2024-02-12T14:52:53Z
dc.date.available 2024-02-12T14:52:53Z
dc.date.created 2019-09
dc.date.issued 2019-09
dc.identifier.uri https://hdl.handle.net/20.500.12080/39726
dc.description.abstract HIV remains incurable because of viral persistence in latent reservoirs that are inaccessible to antiretroviral therapy. A potential curative strategy is to reactivate viral gene expression in latently infected cells. However, no drug so far has proven to be successful in vivo in reducing the reservoir, and therefore new anti-latency compounds are needed. We explored the role of microRNAs (miRNAs) in latency maintenance and their modulation as a potential anti-latency strategy. Latency models based on treating resting CD4 T cells with chemokine (C-C motif) ligand 19 (CCL19) or interleukin-7 (IL7) before HIV infection and next-generation sequencing were used to identify the miRNAs involved in HIV latency. We detected four upregu lated miRNAs (miRNA-98, miRNA-4516, miRNA-4488, and miRNA-7974). Individual or combined inhibition of these miRNAs was performed by transfection into cells latently infected with HIV. Viral replication, assessed 72 h after trans fection, did not increase after miRNA modulation, despite miRNA inhibition and lack of toxicity. Furthermore, the com bined modulation of five miRNAs previously associated with HIV latency was not effective in these models. Our results do not support the modulation of miRNAs as a useful strategy for the reversal of HIV latency. As shown with other drugs, the potential of miRNA modulation as an HIV reactivation strategy could be dependent on the latency model used. es_ES
dc.format application/pdf es_ES
dc.language eng es_ES
dc.rights CC-BY es_ES
dc.rights.uri http://creativecommons.org/licenses/by/4.0/deed.es es_ES
dc.title Selective miRNA Modulation Fails to Activate HIV Replication in In Vitro Latency Models es_ES
dc.type info:eu-repo/semantics/article es_ES
dc.rights.accessrights info:eu-repo/semantics/openAccess es_ES
dc.identifier.location N/A es_ES


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