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Blood Clearance and Activity of Erythrocyte-Coupled Fibrinolytics

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Ganguly, Kumkum & Krasik, Tatiana & Medinilla, Sandra & Bdeir, Khalil & Cines, Douglas B. & Muzykantov, Vladimir R. & Murciano, Juan Carlos (2005 ) .Blood Clearance and Activity of Erythrocyte-Coupled Fibrinolytics.

ISO 690

Ganguly, Kumkum & Krasik, Tatiana & Medinilla, Sandra & Bdeir, Khalil & Cines, Douglas B. & Muzykantov, Vladimir R. & Murciano, Juan Carlos. 2005 .Blood Clearance and Activity of Erythrocyte-Coupled Fibrinolytics.

https://hdl.handle.net/20.500.12080/39706
dc.contributor.author Ganguly, Kumkum
dc.contributor.author Krasik, Tatiana
dc.contributor.author Medinilla, Sandra
dc.contributor.author Bdeir, Khalil
dc.contributor.author Cines, Douglas B.
dc.contributor.author Muzykantov, Vladimir R.
dc.contributor.author Murciano, Juan Carlos
dc.date.accessioned 2024-02-12T10:29:06Z
dc.date.available 2024-02-12T10:29:06Z
dc.date.created 2005
dc.date.issued 2005
dc.identifier.uri https://hdl.handle.net/20.500.12080/39706
dc.description.abstract blood cells (RBCs) endows it with features useful for thrombo prophylaxis. However, the optimal intensity and duration of thromboprophylaxis vary among clinical settings. To assess how the intrinsic properties of a plasminogen activator (PA) affect functions of the corresponding RBC/PA conjugate, we coupled equal amounts of tPA or Retavase (rPA; a variant with an extended circulation time, lower fibrin affinity, and greater susceptibility to PA inhibitors). Conjugation to RBC markedly prolonged the circulation of each PA in rats and mice, without detrimental effects on carrier RBC. The initial blood clearance of RBC/tPA was faster than RBC/rPA, yet it exerted greater fibrinolytic activity, in part due to greater resistance of tPA and RBC/tPA to plasma inhibitors versus rPA and RBC/rPA ob served in vitro. Soluble and RBC-coupled tPA and rPA exerted the same amidolytic activity, yet RBC/tPA lysed fibrin clots more effectively than RBC/rPA, notwithstanding comparable fibrinolytic activity of their soluble counterparts. Conjugation to RBC suppressed rPA¿s ability to be activated by fibrin, whereas the fibrin activation of RBC-coupled tPA was not hindered. Therefore, the functional profile of RBC/PA is influenced by: pharmacokinetic features provided by carrier RBC (e.g., prolonged circulation), intrinsic PA features (e.g., clearance rate, resistance to inhibitors), and changes imposed by conjugation to RBC (e.g., loss of cofactor stim es_ES
dc.format application/pdf es_ES
dc.language eng es_ES
dc.rights CC-BY es_ES
dc.rights.uri http://creativecommons.org/licenses/by/4.0/deed.es es_ES
dc.title Blood Clearance and Activity of Erythrocyte-Coupled Fibrinolytics es_ES
dc.type info:eu-repo/semantics/article es_ES
dc.rights.accessrights info:eu-repo/semantics/openAccess es_ES
dc.identifier.location N/A es_ES


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