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Detection of virulence-associated genes characteristic of intestinal Escherichia coli pathotypes, including the enterohemorrhagic/enteroaggregative O104:H4, in bovines from Germany and Spain

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Cabal, Adriana & Geue, Lutz & Gómez Barrero, Susana & Barth, Stefanie & Barcena, Carmen & Hamm, Katharina & Porrero, M.Concepcion & Valverde, Aranzazu & Canton, Rafael & Menge, Christian & Gortazar, Christian & Domínguez, Lucas & Alvarez, Julio (2015 ) .Detection of virulence-associated genes characteristic of intestinal Escherichia coli pathotypes, including the enterohemorrhagic/enteroaggregative O104:H4, in bovines from Germany and Spain.

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Cabal, Adriana & Geue, Lutz & Gómez Barrero, Susana & Barth, Stefanie & Barcena, Carmen & Hamm, Katharina & Porrero, M.Concepcion & Valverde, Aranzazu & Canton, Rafael & Menge, Christian & Gortazar, Christian & Domínguez, Lucas & Alvarez, Julio. 2015 .Detection of virulence-associated genes characteristic of intestinal Escherichia coli pathotypes, including the enterohemorrhagic/enteroaggregative O104:H4, in bovines from Germany and Spain.

https://hdl.handle.net/20.500.12080/39630
dc.contributor.author Cabal, Adriana
dc.contributor.author Geue, Lutz
dc.contributor.author Gómez Barrero, Susana
dc.contributor.author Barth, Stefanie
dc.contributor.author Barcena, Carmen
dc.contributor.author Hamm, Katharina
dc.contributor.author Porrero, M.Concepcion
dc.contributor.author Valverde, Aranzazu
dc.contributor.author Canton, Rafael
dc.contributor.author Menge, Christian
dc.contributor.author Gortazar, Christian
dc.contributor.author Domínguez, Lucas
dc.contributor.author Alvarez, Julio
dc.date.accessioned 2024-02-09T09:20:03Z
dc.date.available 2024-02-09T09:20:03Z
dc.date.created 2015
dc.date.issued 2015
dc.identifier.uri https://hdl.handle.net/20.500.12080/39630
dc.description.abstract Cattle are reservoirs of enterohemorrhagic Escherichia coli; however, their role in the epidemiology of other pathogenic E. coli remains undefined. A new set of quantitative real-time PCR assays for the direct detection and quantification of nine virulence-associated genes (VAGs) characteristic of the most important human E. coli pathotypes and four serotype-related genes (wzxO104,fliCH4,rbfO157, fliCH7) that can be used as a surveillance tool for detection of pathogenic strains was developed. A total of 970 cattle fecal samples were collected in slaughterhouses in Germany and Spain, pooled into 134 samples and analyzed with this tool. stx1, eae and invA were more prevalent in Spanish samples whereas bfpA, stx2, ehxA, elt, est and the rbfO157/fliCH7 combination were observed in similar proportions in both countries. Genes characteristic of the hybrid O104:H4 strain of the 2011 German outbreak (stx2/aggR/wzxO104/ fliCH4) were simultaneously detected in six fecal pools from one German abattoir located near the outbreak epicenter. Although no isolate harboring the full stx2/aggR/wzxO104/fliCH4 combination was cultured, sequencing of the aggR positive PCR products revealed 100% homology to the aggR from the outbreak strain. Concomitant detection by this direct approach of VAGs from a novel human pathogenic E. coli strain in cattle samples implies that the E. coli gene pool in these animals can be implicated in de novo formation of such highly-virulent strains. The application of this set of qPCRs in surveillance studies could be an efficient early-warning tool for the emergence of zoonotic E. coli in livestock. Key words Escherichia coli, virulence-associated genes, pathotypes, O104:H4. es_ES
dc.format application/pdf es_ES
dc.language eng es_ES
dc.rights CC-BY es_ES
dc.rights.uri http://creativecommons.org/licenses/by/4.0/deed.es es_ES
dc.title Detection of virulence-associated genes characteristic of intestinal Escherichia coli pathotypes, including the enterohemorrhagic/enteroaggregative O104:H4, in bovines from Germany and Spain es_ES
dc.type info:eu-repo/semantics/article es_ES
dc.rights.accessrights info:eu-repo/semantics/openAccess es_ES
dc.identifier.location N/A es_ES


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