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Efficacy of a typing scheme for Campylobacter based on the combination of true and questionable CRISPR

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APA

de Cárdenas, Inés & Fernández Garayzábal, José F. & de la Cruz, María Luisa & Domínguez, Lucas & Ugarte Ruiz, María & Gómez Barrero, Susana (2015 ) .Efficacy of a typing scheme for Campylobacter based on the combination of true and questionable CRISPR.

ISO 690

de Cárdenas, Inés & Fernández Garayzábal, José F. & de la Cruz, María Luisa & Domínguez, Lucas & Ugarte Ruiz, María & Gómez Barrero, Susana. 2015 .Efficacy of a typing scheme for Campylobacter based on the combination of true and questionable CRISPR.

https://hdl.handle.net/20.500.12080/39608
dc.contributor.author de Cárdenas, Inés
dc.contributor.author Fernández Garayzábal, José F.
dc.contributor.author de la Cruz, María Luisa
dc.contributor.author Domínguez, Lucas
dc.contributor.author Ugarte Ruiz, María
dc.contributor.author Gómez Barrero, Susana
dc.date.accessioned 2024-02-08T16:14:21Z
dc.date.available 2024-02-08T16:14:21Z
dc.date.created 2015
dc.date.issued 2015
dc.identifier.uri https://hdl.handle.net/20.500.12080/39608
dc.description.abstract This study evaluates an improved scheme for Campylobacter genotyping based on the combination of true and questionable CRISPR (clustered regularly interspaced short palindromic repeats) elements. A total of 180 Campylobacter strains (Campylobacter jejuni n = 93 and Campylobacter coli n = 87), isolated from neck skin and caecal content of broilers, poultry meat and sewage water were analysed. Another 97 C. jejuni DNA samples from cases of human campylobacteriosis were assessed. Sixty-three genotypes were found in C. jejuni considering only true CRISPR, and 16 additional genotypes were identified when questionable CRISPR were also taken into account. Likewise in C. coli the number of genotypes increased from eight for only true CRISPR to 14 after in cluding questionable CRISPR elements. The number of typeable C. jejuni and C. coli isolates was 115 (60.5%) and 17 (19.5%) respectively considering only true CRISPR. These percentages increased to 92.7% (n = 176) and 39.1% (n = 34) respectively when both true and questionable CRISPR were considered. 60.9% of the C. coli isolates were non-typeable by CRISPR due to the lack of any PCR amplifiable CRISPR loci, which raises questions about CRISPR analysis as an appropriate method for C. coli typing. However the assessment of true and questionable CRISPR has proved to be fairly useful for typing C. jejuni due to its high discriminatory power (Simpson's index = 0.960) and typeability (92.7%) values. The results of the present work show that our genotyping method based on the com bination of true and questionable CRISPR elements may be used as a suitable complementary tool to existing C. jejuni genotyping methods. es_ES
dc.format application/pdf es_ES
dc.language eng es_ES
dc.rights CC-BY es_ES
dc.rights.uri http://creativecommons.org/licenses/by/4.0/deed.es es_ES
dc.title Efficacy of a typing scheme for Campylobacter based on the combination of true and questionable CRISPR es_ES
dc.type info:eu-repo/semantics/article es_ES
dc.rights.accessrights info:eu-repo/semantics/restrictedAccess es_ES
dc.identifier.location N/A es_ES


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