Development of an optimized ELISA and a sample preparation method for the detection  ¿-actoglobulin traces in baby foods

Pelaez Lorenzo, Cristina; Diez Masa, Jose Carlos; Vasallo, I.; De Frutos, Mercedes

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APA

Pelaez Lorenzo, Cristina & Diez Masa, Jose Carlos & Vasallo, I. & De Frutos, Mercedes (2010-01 ) .Development of an optimized ELISA and a sample preparation method for the detection ¿-actoglobulin traces in baby foods.

ISO 690

Pelaez Lorenzo, Cristina & Diez Masa, Jose Carlos & Vasallo, I. & De Frutos, Mercedes. 2010-01 .Development of an optimized ELISA and a sample preparation method for the detection ¿-actoglobulin traces in baby foods.

https://hdl.handle.net/20.500.12080/39604

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dc.contributor.author	Pelaez Lorenzo, Cristina
dc.contributor.author	Diez Masa, Jose Carlos
dc.contributor.author	Vasallo, I.
dc.contributor.author	De Frutos, Mercedes
dc.date.accessioned	2024-02-08T15:19:40Z
dc.date.available	2024-02-08T15:19:40Z
dc.date.created	2010-01
dc.date.issued	2010-01
dc.identifier.uri	https://hdl.handle.net/20.500.12080/39604
dc.description.abstract	Dairy products can induce allergic reactions even when present at very low levels, such as levels found in involuntary contamination during food manufacturing. ¿-Lactoglobulin (¿LG) is the main allergen in cow's milk. The objective of this work was to develop a sensitive method for ¿LG detection in baby foods through the optimization of an innovative sample preparation method. Three types of baby foods deliberately contaminated with dairy products or dairy desserts were sterilized to simulate the potential contamination occurring during manufacturing and then used as samples. Different sample preparation methods were compared. The best results were provided by an extraction solution containing ¿-mercaptoethanol, guanidine hydrochloride, and a saline solution. An ELISA method was optimized for the detection of ¿LG (LOD = 9.7 × 10-13 M). The developed method allowed detection of even 1 part of dairy product in 100,000 parts of baby food for some of the analyzed foods. © 2010 American Chemical Society.	es_ES
dc.format	application/pdf	es_ES
dc.language	eng	es_ES
dc.rights	CC-BY	es_ES
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/deed.es	es_ES
dc.title	Development of an optimized ELISA and a sample preparation method for the detection ¿-actoglobulin traces in baby foods	es_ES
dc.type	info:eu-repo/semantics/article	es_ES
dc.rights.accessrights	info:eu-repo/semantics/restrictedAccess	es_ES
dc.identifier.location	N/A	es_ES