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Development of an optimized ELISA and a sample preparation method for the detection ¿-actoglobulin traces in baby foods

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APA

Pelaez Lorenzo, Cristina & Diez Masa, Jose Carlos & Vasallo, I. & De Frutos, Mercedes (2010-01 ) .Development of an optimized ELISA and a sample preparation method for the detection ¿-actoglobulin traces in baby foods.

ISO 690

Pelaez Lorenzo, Cristina & Diez Masa, Jose Carlos & Vasallo, I. & De Frutos, Mercedes. 2010-01 .Development of an optimized ELISA and a sample preparation method for the detection ¿-actoglobulin traces in baby foods.

https://hdl.handle.net/20.500.12080/39604
dc.contributor.author Pelaez Lorenzo, Cristina
dc.contributor.author Diez Masa, Jose Carlos
dc.contributor.author Vasallo, I.
dc.contributor.author De Frutos, Mercedes
dc.date.accessioned 2024-02-08T15:19:40Z
dc.date.available 2024-02-08T15:19:40Z
dc.date.created 2010-01
dc.date.issued 2010-01
dc.identifier.uri https://hdl.handle.net/20.500.12080/39604
dc.description.abstract Dairy products can induce allergic reactions even when present at very low levels, such as levels found in involuntary contamination during food manufacturing. ¿-Lactoglobulin (¿LG) is the main allergen in cow's milk. The objective of this work was to develop a sensitive method for ¿LG detection in baby foods through the optimization of an innovative sample preparation method. Three types of baby foods deliberately contaminated with dairy products or dairy desserts were sterilized to simulate the potential contamination occurring during manufacturing and then used as samples. Different sample preparation methods were compared. The best results were provided by an extraction solution containing ¿-mercaptoethanol, guanidine hydrochloride, and a saline solution. An ELISA method was optimized for the detection of ¿LG (LOD = 9.7 × 10-13 M). The developed method allowed detection of even 1 part of dairy product in 100,000 parts of baby food for some of the analyzed foods. © 2010 American Chemical Society. es_ES
dc.format application/pdf es_ES
dc.language eng es_ES
dc.rights CC-BY es_ES
dc.rights.uri http://creativecommons.org/licenses/by/4.0/deed.es es_ES
dc.title Development of an optimized ELISA and a sample preparation method for the detection ¿-actoglobulin traces in baby foods es_ES
dc.type info:eu-repo/semantics/article es_ES
dc.rights.accessrights info:eu-repo/semantics/restrictedAccess es_ES
dc.identifier.location N/A es_ES


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