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Implication of Polycomb Members Bmi-1, Mel-18, and Hpc-2 in the Regulation of p16INK4a, p14ARF, h-TERT, and c-Myc Expression in Primary Breast Carcinomas

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APA

Silva, Javier & García, José M. & Peña, Cristina & García, Vanesa & Domínguez, Gemma & Suarez, Dolores & Camacho, Francisca I. & Espinosa, Ruth & Provencio, Mariano & España, Pilar & Bonilla, Félix (2006-12 ) .Implication of Polycomb Members Bmi-1, Mel-18, and Hpc-2 in the Regulation of p16INK4a, p14ARF, h-TERT, and c-Myc Expression in Primary Breast Carcinomas.

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Silva, Javier & García, José M. & Peña, Cristina & García, Vanesa & Domínguez, Gemma & Suarez, Dolores & Camacho, Francisca I. & Espinosa, Ruth & Provencio, Mariano & España, Pilar & Bonilla, Félix. 2006-12 .Implication of Polycomb Members Bmi-1, Mel-18, and Hpc-2 in the Regulation of p16INK4a, p14ARF, h-TERT, and c-Myc Expression in Primary Breast Carcinomas.

https://hdl.handle.net/20.500.12080/39573
dc.contributor.author Silva, Javier
dc.contributor.author García, José M.
dc.contributor.author Peña, Cristina
dc.contributor.author García, Vanesa
dc.contributor.author Domínguez, Gemma
dc.contributor.author Suarez, Dolores
dc.contributor.author Camacho, Francisca I.
dc.contributor.author Espinosa, Ruth
dc.contributor.author Provencio, Mariano
dc.contributor.author España, Pilar
dc.contributor.author Bonilla, Félix
dc.date.accessioned 2024-02-08T12:01:56Z
dc.date.available 2024-02-08T12:01:56Z
dc.date.created 2006-12
dc.date.issued 2006-12
dc.identifier.uri https://hdl.handle.net/20.500.12080/39573
dc.description.abstract Purpose: Deregulation of mammalian Polycomb group (PcG) members may contribute to human carcinogenesis. p16INK4a and p14ARF tumor suppressors, human telomerase reverse transcriptase (h-TERT), and oncoprotein c-Myc have been implicated in the regulation of the cell cycle and proliferation mediated by PcG proteins, mainly Bmi-1, in mice and in cell culture experiments. Here, we examine whether these in vitro findings can be extrapolated to the in vivo situation. Experimental Design:We measure the expression of PcG members Bmi-1, Mel-18, and Hpc-2 and their potential targets by reverse transcription-PCR, immunostaining, andWestern blotting in a series of 134 breast carcinomas and correlate the data with several clinical-pathologic variables of the tumors. Results: Expression of PcG genes was variably detected, but overexpression of Bmi-1 was the most frequent PcG alteration observed. In addition, statistical direct correlation in expression level of the three PcG members was detected. A correlation between c-Myc and Bmi-1 expression levels was observed; however, there was no correlation between expression of Bmi-1 and p16INK4a, p14ARF, or h-TERT. However, expression of the other PcG members Mel-18 and Hpc-2 correlated withthe cell cycle regulators. Moreover, PcG mRNA ^ altered expression correlated significantly withcertain clinical-pathologic variables associated withpoor prognosis. Conclusions: Our data suggest that the oncogenic role of Bmi-1 in human primary breast carcinomas is not determined by its capacity to inhibit INK4a/ARF proteins or to induce telomer ase activity es_ES
dc.format application/pdf es_ES
dc.language eng es_ES
dc.rights CC-BY es_ES
dc.rights.uri http://creativecommons.org/licenses/by/4.0/deed.es es_ES
dc.title Implication of Polycomb Members Bmi-1, Mel-18, and Hpc-2 in the Regulation of p16INK4a, p14ARF, h-TERT, and c-Myc Expression in Primary Breast Carcinomas es_ES
dc.type info:eu-repo/semantics/article es_ES
dc.rights.accessrights info:eu-repo/semantics/openAccess es_ES
dc.identifier.location N/A es_ES


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