Repositorio Institucional de la Universidad Alfonso X el Sabio

A natural antisense transcript regulates Zeb2/Sip1 gene expression during Snail1-induced epithelial¿mesenchymal transition

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APA

Beltran, Manuel & Puig, Isabel & Peña, Cristina & García, José Miguel & Álvarez, Ana Belén & Peña, Raúl & Bonilla, Felix & García de Herreros, Antonio (2008 ) .A natural antisense transcript regulates Zeb2/Sip1 gene expression during Snail1-induced epithelial¿mesenchymal transition.

ISO 690

Beltran, Manuel & Puig, Isabel & Peña, Cristina & García, José Miguel & Álvarez, Ana Belén & Peña, Raúl & Bonilla, Felix & García de Herreros, Antonio. 2008 .A natural antisense transcript regulates Zeb2/Sip1 gene expression during Snail1-induced epithelial¿mesenchymal transition.

https://hdl.handle.net/20.500.12080/39560
dc.contributor.author Beltran, Manuel
dc.contributor.author Puig, Isabel
dc.contributor.author Peña, Cristina
dc.contributor.author García, José Miguel
dc.contributor.author Álvarez, Ana Belén
dc.contributor.author Peña, Raúl
dc.contributor.author Bonilla, Felix
dc.contributor.author García de Herreros, Antonio
dc.date.accessioned 2024-02-08T08:42:16Z
dc.date.available 2024-02-08T08:42:16Z
dc.date.created 2008
dc.date.issued 2008
dc.identifier.uri https://hdl.handle.net/20.500.12080/39560
dc.description.abstract Expression of Snail1 in epithelial cells triggers an epithelial¿mesenchymal transition (EMT). Here, we demonstrate that the synthesis of Zeb2, a transcriptional repressor of E-cadherin, is up-regulated after Snail1-induced EMT. Snail1 does not affect the synthesis of Zeb2 mRNA, but prevents the processing of a large intron located in its 5 -untranslated region (UTR). This intron contains an internal ribosome entry site (IRES) necessary for the expression of Zeb2. Maintenance of 5 -UTR Zeb2 intron is dependent on the expression of a natural antisense transcript (NAT) that overlaps the 5 splice site in the intron. Ectopic overexpression of this NAT in epithelial cells prevents splicing of the Zeb2 5 -UTR, increases the levels of Zeb2 protein, and consequently down-regulates E-cadherin mRNA and protein. The relevance of these results is demonstrated by the strong association between NAT presence and conservation of the 5 -UTR intron in cells that have undergone EMT or in human tumors with low E-cadherin expression. Therefore, the results presented in this article reveal the existence of a NAT capable of activating Zeb2 expression, explain the mechanism involved in this activation, and demonstrate that this NAT regulates E-cadherin expression. es_ES
dc.format application/pdf es_ES
dc.language eng es_ES
dc.rights CC-BY es_ES
dc.rights.uri http://creativecommons.org/licenses/by/4.0/deed.es es_ES
dc.source GENES & DEVELOPMENT es_ES
dc.title A natural antisense transcript regulates Zeb2/Sip1 gene expression during Snail1-induced epithelial¿mesenchymal transition es_ES
dc.type info:eu-repo/semantics/article es_ES
dc.rights.accessrights info:eu-repo/semantics/openAccess es_ES
dc.identifier.location N/A es_ES


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