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Chemotaxis in Leishmania (Viannia) braziliensis: Evaluation by the two-chamber capillary assay

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Díaz López, Emilia & Ríos Díaz, Arturo & Vanegas Calderón, Oriana & Lajkó, Eszter & Ponte-Sucre, Alicia & Kohidai, László (2021-01 ) .Chemotaxis in Leishmania (Viannia) braziliensis: Evaluation by the two-chamber capillary assay.

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Díaz López, Emilia & Ríos Díaz, Arturo & Vanegas Calderón, Oriana & Lajkó, Eszter & Ponte-Sucre, Alicia & Kohidai, László. 2021-01 .Chemotaxis in Leishmania (Viannia) braziliensis: Evaluation by the two-chamber capillary assay.

https://hdl.handle.net/20.500.12080/39504
dc.contributor.author Díaz López, Emilia
dc.contributor.author Ríos Díaz, Arturo
dc.contributor.author Vanegas Calderón, Oriana
dc.contributor.author Lajkó, Eszter
dc.contributor.author Ponte-Sucre, Alicia
dc.contributor.author Kohidai, László
dc.date.accessioned 2024-02-06T11:12:09Z
dc.date.available 2024-02-06T11:12:09Z
dc.date.created 2021-01
dc.date.issued 2021-01
dc.identifier.uri https://hdl.handle.net/20.500.12080/39504
dc.description.abstract Chemotactic responses play a significant role during Leishmania (V.) braziliensis differentiation through its life cycle and during infection. The aim of this description has been to portray the modified ¿two-chamber capillary chemotaxis assay¿ as a technique useful for quantitative in vitro evaluation of Leishmania chemotaxis after reviewing the methods described until now to assess chemotaxis in vitro in Leishmania sp. This valued simple and reproducible method convenient for parasite migration determination, was tested by the use of controlled changes in monosaccharide (D-glucose and D-fructose) concentrations as referent ligands. The validation of the method demonstrates that this technique is useful to evaluate the relationship existing between parasite migration towards the monosaccharides and sugar concentration. This means that within specific ranges, parasites attracted by the monosaccharide migrate towards more concentrated solutions and accumulate (higher number of parasites) at that spot. Interestingly, both the time course of the experiment and the osmolality of the solution influence parasite migration capacity. Our validation suggests that this improved methodology quantitatively evaluates taxis of Leishmania towards/against different substances. On the basis of our herein presented data, we conclude that this technique is a novel, rapid and reliable screening method to evaluate chemotaxis in Leishmania. es_ES
dc.format application/pdf es_ES
dc.language eng es_ES
dc.rights CC-BY es_ES
dc.rights.uri http://creativecommons.org/licenses/by/4.0/deed.es es_ES
dc.title Chemotaxis in Leishmania (Viannia) braziliensis: Evaluation by the two-chamber capillary assay es_ES
dc.type info:eu-repo/semantics/article es_ES
dc.rights.accessrights info:eu-repo/semantics/openAccess es_ES
dc.identifier.location N/A es_ES


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