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Is the activity of CGRP and Adrenomedullin regulated by RAMP (-2) and (-3) in Trypanosomatidae? An in-silico approach

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Díaz López, Emilia & Febres, Anthony & Vanegas, Oriana & Giammarresi, Michelle & Gomes, Carlos & Ponte-Sucre, Alicia (2018 ) .Is the activity of CGRP and Adrenomedullin regulated by RAMP (-2) and (-3) in Trypanosomatidae? An in-silico approach.

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Díaz López, Emilia & Febres, Anthony & Vanegas, Oriana & Giammarresi, Michelle & Gomes, Carlos & Ponte-Sucre, Alicia. 2018 .Is the activity of CGRP and Adrenomedullin regulated by RAMP (-2) and (-3) in Trypanosomatidae? An in-silico approach.

https://hdl.handle.net/20.500.12080/39376
dc.contributor.author Díaz López, Emilia
dc.contributor.author Febres, Anthony
dc.contributor.author Vanegas, Oriana
dc.contributor.author Giammarresi, Michelle
dc.contributor.author Gomes, Carlos
dc.contributor.author Ponte-Sucre, Alicia
dc.date.accessioned 2024-01-26T12:10:25Z
dc.date.available 2024-01-26T12:10:25Z
dc.date.created 2018
dc.date.issued 2018
dc.identifier.uri https://hdl.handle.net/20.500.12080/39376
dc.description.abstract The Calcitonin-Like Receptor (CLR) belongs to the classical seven-transmembrane segment molecules coupled to heterotrimeric G proteins. Its pharmacology depends on the simultaneous expression of the so-called Receptor Activity Modifier Proteins (RAMP-) ¿1, ¿2 and ¿3. RAMP-associated proteins modulate glycosylation and cellular traffic of CLR, therefore determining its pharmacodynamics. In higher eukaryotes, the complex formed by CLR and RAMP-1 is more akin to bind Calcitonin Gene-Related Peptide (CGRP), whereas those formed by CLR and RAMP-2 or RAMP-3, bind preferentially Adrenomedullin (AM). In lower eukaryotes, RAMPs, or any homologous protein, have not been identified until now. Herein we demonstrated a negative chemotactic response elicited by CGRP (10¿9 and 10¿8 M) and AM (10¿9 to 10¿5 M). Whether or not this response is receptor mediated should be verified, as well as the expression of a 24 kDa band in Leishmania, recognized by western blot analysis by the use of (human-)-RAMP-2 antibodies as detection probes. Queries with human RAMP-2 and RAMP-3 protein sequences in blastp against Leishmania (Viannia) braziliensis predicted proteome, allowed us to detect two sequence alignments in the parasite: A RAMP-2-aligned sequence corresponding to Leishmania folylpolyglutamate synthase (FPGS), and a RAMP-3 aligned protein, a hypothetical Leishmania protein with yet unknown function. The presence of homologous of these proteins was described in-silico in other members of the Trypanosomatidae. These preliminary and not yet complete data suggest the feasibility that both CGRP and Adrenomedullin activities may be regulated by homologs of RAMP- (¿2) and (¿3) in these parasites. es_ES
dc.format application/pdf es_ES
dc.language eng es_ES
dc.rights CC-BY es_ES
dc.rights.uri http://creativecommons.org/licenses/by/4.0/deed.es es_ES
dc.title Is the activity of CGRP and Adrenomedullin regulated by RAMP (-2) and (-3) in Trypanosomatidae? An in-silico approach es_ES
dc.type info:eu-repo/semantics/article es_ES
dc.rights.accessrights info:eu-repo/semantics/openAccess es_ES
dc.identifier.location N/A es_ES


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