APA
Rodrigo Muñoz, José M. & Gil Martínez, Marta & Lorente Sorolla, Clara & Sastre, Beatriz & García García, María Luz & Calvo Rey, Cristina & Casas, Inmaculada & del Pozo, Victoria .Reduced miR-146a-5p Is a Biomarker of Infant Respiratory Diseases Contributing to Immune Dysregulation in Small Airway Epithelial Cells.
ISO 690
Rodrigo Muñoz, José M. & Gil Martínez, Marta & Lorente Sorolla, Clara & Sastre, Beatriz & García García, María Luz & Calvo Rey, Cristina & Casas, Inmaculada & del Pozo, Victoria. Reduced miR-146a-5p Is a Biomarker of Infant Respiratory Diseases Contributing to Immune Dysregulation in Small Airway Epithelial Cells.
https://hdl.handle.net/20.500.12080/31858
Resumen:
Respiratory diseases such as bronchiolitis, and those with wheezing episodes, are highly
important during infancy due to their potential chronicity. Immune response dysregulation is critical in perpetuating lung damage. Epigenetic modifications including microRNA (miRNA) posttranscriptional regulation are among the factors involved in alleviating inflammation. We evaluated
the expression of miR-146a-5p, a previously described negative regulator of immunity, in infants
with respiratory diseases, in order to study epigenetic regulation of the immune response. Nasopharyngeal aspirate (NPA) was obtained from infants with bronchiolitis (ongoing and post-disease)
or with wheezing episodes in addition to healthy controls. Virus presence was determined by
nested PCR, while miRNA and gene expression were studied in cells from NPAs using qPCR.
Healthy small airway epithelial cells (SAECs) were used as an in vitro model. We observe a reduction in miR-146a-5p expression in infants with either of the two diseases compared to controls, suggesting the potential of this miRNA as a disease biomarker. Post-bronchiolitis, miR-146a-5p expression increases, though without reaching levels of healthy controls. MiR-146a-5p expression correlates inversely with the immune-related gene PTGS2, while its expression correlates directly with
TSLP. When heathy donor SAECs are stimulated by poly:IC, we observe an increase in miR-146a5p, with wounds having a synergistic effect. In conclusion, infants with respiratory diseases present
reduced miR-146a-5p expression, possibly affecting immune dysregulation.
Keywords: infant respiratory diseases; microRNAs; immune regulation