APA
Valle Millares, Daniel & Brochado Kith, Óscar & Martín Carbonero, Luz & Domínguez Domínguez, Lourdes & Ryan, Pablo & De los Santos, Ignacio & De la Fuente, Sara & Castro, Juan M. & Lagarde, María & Cuevas, Guillermo & Mayoral Muñoz, Mario & Matarranz, Mariano & Díez, Victorino & Gómez Sanz, Alicia & Martínez Román, Paula & Crespo Bermejo, Celia & Palladino, Claudia & Muñoz Muñoz, María & Jiménez Sousa, María A. & Resino, Salvador & Briz, Verónica & Fernández Rodríguez, Amanda .Different HCV Exposure Drives Specific miRNA Profile in PBMCs of HIV Patients.
ISO 690
Valle Millares, Daniel & Brochado Kith, Óscar & Martín Carbonero, Luz & Domínguez Domínguez, Lourdes & Ryan, Pablo & De los Santos, Ignacio & De la Fuente, Sara & Castro, Juan M. & Lagarde, María & Cuevas, Guillermo & Mayoral Muñoz, Mario & Matarranz, Mariano & Díez, Victorino & Gómez Sanz, Alicia & Martínez Román, Paula & Crespo Bermejo, Celia & Palladino, Claudia & Muñoz Muñoz, María & Jiménez Sousa, María A. & Resino, Salvador & Briz, Verónica & Fernández Rodríguez, Amanda. Different HCV Exposure Drives Specific miRNA Profile in PBMCs of HIV Patients.
https://hdl.handle.net/20.500.12080/26278
Resumen:
Micro RNAs (miRNAs) are essential players in HIV and HCV infections, as both viruses
modulate cellular miRNAs and interact with the miRNA-mediated host response. We aim to analyze
the miRNA profile of HIV patients with different exposure to HCV to explore specific signatures in the
miRNA profile of PBMCs for each type of infection. We massively sequenced small RNAs of PBMCs
from 117 HIV+ infected patients: 45 HIV+ patients chronically infected with HCV (HIV/HCV+),
36 HIV+ that spontaneously clarified HCV after acute infection (HIV/HCV-) and 36 HIV+ patients
without previous HCV infection (HIV). Thirty-two healthy patients were used as healthy controls
(HC). Differential expression analysis showed significantly differentially expressed (SDE) miRNAs
in HIV/HCV+ (n = 153), HIV/HCV- (n = 169) and HIV (n = 153) patients. We found putative
dysregulated pathways, such as infectious-related and PI3K signaling pathways, common in all
contrasts. Specifically, putatively targeted genes involved in antifolate resistance (HIV/HV+), cancerrelated pathways (HIV/HCV-) and HIF-signaling (HIV) were identified, among others. Our findings
revealed that HCV strongly influences the expression profile of PBMCs from HIV patients through
the disruption of its miRNome. Thus, different HCV exposure can be identified by specific miRNA
signatures in PBMCs.