EVIDENCE FOR SIMULTANEOUS 1Na+ :1Mg2+ AND PING PONG 2Na+ :1Mg2+ EXCHANGERS IN RAT THYMOCYTES

Contreras Jurado, Silvia Constanza; Sanchez Morito, Nuria; Ruiz Contreras, Anabel; Gonzalez Martinez, Marco T.; Soler Diaz, Agatangelo

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Contreras Jurado, Silvia Constanza & Sanchez Morito, Nuria & Ruiz Contreras, Anabel & Gonzalez Martinez, Marco T. & Soler Diaz, Agatangelo .EVIDENCE FOR SIMULTANEOUS 1Na+ :1Mg2+ AND PING PONG 2Na+ :1Mg2+ EXCHANGERS IN RAT THYMOCYTES.

ISO 690

Contreras Jurado, Silvia Constanza & Sanchez Morito, Nuria & Ruiz Contreras, Anabel & Gonzalez Martinez, Marco T. & Soler Diaz, Agatangelo. EVIDENCE FOR SIMULTANEOUS 1Na+ :1Mg2+ AND PING PONG 2Na+ :1Mg2+ EXCHANGERS IN RAT THYMOCYTES.

https://hdl.handle.net/20.500.12080/26189

EndNote

Mendeley

dc.contributor.author	Contreras Jurado, Silvia Constanza
dc.contributor.author	Sanchez Morito, Nuria
dc.contributor.author	Ruiz Contreras, Anabel
dc.contributor.author	Gonzalez Martinez, Marco T.
dc.contributor.author	Soler Diaz, Agatangelo
dc.date.accessioned	2021-11-10T17:11:22Z
dc.date.available	2021-11-10T17:11:22Z
dc.date.created	2005-05-01
dc.identifier.uri	https://hdl.handle.net/20.500.12080/26189
dc.description.tableofcontents	Rat thymocytes showed two Na+ /Mg2+ exchangers with high- and low- affinities for external Na+ (Na+ o) at physiological internal Mg2+content. The total internal Mg2+ content (Mg2+it) was enhanced by loading with MgCl2 and the ionophore A-23187. Under these conditions, Na+ /Mg2+ exchangers were dramatically stimulated by the Mg2+it increase. Na+ -induced Mg2+ effluxes were independent of Cl o or H+ . The Na+ /Mg2+ exchangers, which we named HANao (high affinity for Na+ o) and LANao (low affinity for Na+ o), were dissected in Mg2+-loaded thymocytes according to their kinetics and stoichiometries. HANao, which showed an apparent dissociation constant for Na+ o (KNa H) = 9.2 +/- 1.6 mmol l -1 Na+ o and a maximal Na+ influx rate (VNa(Na H)max) = 30.5 +/- 6.1 mmol (l cells)-1 h-1, was a 1Na+ :1Mg2+ simultaneous antiporter insensitive to external magnesium (Mg2+o) whereas that LANao, with KNa L = 65.1 +/- 8.6 mmol l-1 Na+ and a VNa(Na L)max = 79.5 +/- 14.3 mmol (l cells)-1 Na+ h-1, was a 2Na+ :1Mg2+ ¿ping-pong¿ antiporter which was strongly inhibited by Mg2+o. At physiological concentration of Mg2+o (1 mM), the Na+ /Mg2+ exchange through the LANao was inhibited by ~50%. Amiloride (10-4 M) inhibited at similar extent both Na+ and Mg2+fluxes at high and at low Na+ o.	es_ES
dc.format	application/pdf	es_ES
dc.language	eng	es_ES
dc.rights	CC-BY	es_ES
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/deed.es	es_ES
dc.title	EVIDENCE FOR SIMULTANEOUS 1Na+ :1Mg2+ AND PING PONG 2Na+ :1Mg2+ EXCHANGERS IN RAT THYMOCYTES	es_ES
dc.type	info:eu-repo/semantics/article	es_ES
dc.rights.accessrights	info:eu-repo/semantics/openAccess	es_ES
dc.identifier.location	N/A	es_ES