APA
Alía García, Esther & Parra Pecharroman, David & Sánchez Díaz, Ana & Mendez, Susy & Royuela Vicente, Ana & Gil Alberdi, Laura & López Palafox, Juan & del Campo Moreno, Rosa .Forensic identification in teeth with caries.
ISO 690
Alía García, Esther & Parra Pecharroman, David & Sánchez Díaz, Ana & Mendez, Susy & Royuela Vicente, Ana & Gil Alberdi, Laura & López Palafox, Juan & del Campo Moreno, Rosa. Forensic identification in teeth with caries.
https://hdl.handle.net/20.500.12080/25962
Resumen:
Human teeth are biological structures that resist extreme conditions thus becoming a useful source of
DNA for human forensic identification purposes. When it is possible, forensic prefer only non-damaged
teeth whereas those with cavities are usually rejected to avoid both external and internal bacterial
contamination. Cavities are one of the most prevalent dental pathology and its incidence increases with
ageing. The aim of this study was to validate the use of teeth with cavities for forensic identification. A
total of 120 individual teeth from unrelated patients (60 healthy and 60 with cavities, respectively)
extracted by a dentist as part of the normal process of treatment, were submitted for further analysis.
Dental pulp was obtained after tooth fragmentation, complete DNA was extracted and the corresponding
human identification profile was obtained by the AmpFlSTR1 NGM SElectTM kit. Cariogenic microbiota
was determined by PCR-DGGE with bacterial universal primers and bands were excised, re-amplified
and sequenced. From the 120 dental pieces analyzed, a defined genetic profile was obtained in 81 (67.5%)
of them, with no statistical differences between the healthy and the cavities-affected teeth. Statistical
association between teeth status, DNA content and genetic profiles was not observed. Complex bacterial
communities were only detected in the cavities group, being the Streptococcus/Enterococcus, and
Lactobacillus genera the most represented. We conclude that teeth with cavities are as valid as healthy
dental pieces for forensic human identification. Moreover, the severity of the cariogenic lesion as well as
associated bacterial communities seems not to influence the establishment of human dental profiles