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Metabolic Alterations Identified in Urine, Plasma and Aortic Smooth Muscle Cells Reflect Cardiovascular Risk in Patients with Programmed Coronary Artery Bypass Grafting

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Santiago Hernandez, Aranzazu & Martinez, Paula J. & Agudiez, Marta & Heredero, Angeles & Gonzalez Calero, Laura & Yuste Montalvo, Alma & Esteban, Vanesa & Aldamiz Echevarria, Gonzalo & Martin Lorenzo, Marta & Alvarez Llamas, Gloria .Metabolic Alterations Identified in Urine, Plasma and Aortic Smooth Muscle Cells Reflect Cardiovascular Risk in Patients with Programmed Coronary Artery Bypass Grafting.

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Santiago Hernandez, Aranzazu & Martinez, Paula J. & Agudiez, Marta & Heredero, Angeles & Gonzalez Calero, Laura & Yuste Montalvo, Alma & Esteban, Vanesa & Aldamiz Echevarria, Gonzalo & Martin Lorenzo, Marta & Alvarez Llamas, Gloria. Metabolic Alterations Identified in Urine, Plasma and Aortic Smooth Muscle Cells Reflect Cardiovascular Risk in Patients with Programmed Coronary Artery Bypass Grafting.

https://hdl.handle.net/20.500.12080/25518
dc.contributor.author Santiago Hernandez, Aranzazu
dc.contributor.author Martinez, Paula J.
dc.contributor.author Agudiez, Marta
dc.contributor.author Heredero, Angeles
dc.contributor.author Gonzalez Calero, Laura
dc.contributor.author Yuste Montalvo, Alma
dc.contributor.author Esteban, Vanesa
dc.contributor.author Aldamiz Echevarria, Gonzalo
dc.contributor.author Martin Lorenzo, Marta
dc.contributor.author Alvarez Llamas, Gloria
dc.date.accessioned 2021-09-07T08:23:52Z
dc.date.available 2021-09-07T08:23:52Z
dc.date.created 2021-08-27
dc.identifier.uri https://hdl.handle.net/20.500.12080/25518
dc.description.abstract Atherosclerosis is the predominant pathology associated to premature deaths due to cardiovascular disease. However, early intervention based on a personalized diagnosis of cardiovascular risk is very limited. We have previously identified metabolic alterations during atherosclerosis development in a rabbit model and in subjects suffering from an acute coronary syndrome. Here we aim to identify specific metabolic signatures which may set the basis for novel tools aiding cardiovascular risk diagnosis in clinical practice. In a cohort of subjects with programmed coronary artery bypass grafting (CABG), we have performed liquid chromatography and targeted mass spectrometry analysis in urine and plasma. The role of vascular smooth muscle cells from human aorta (HA-VSMCs) was also investigated by analyzing the intra and extracellular metabolites in response to a pro-atherosclerotic stimulus. Statistically significant variation was considered if p value < 0.05 (Mann-Whitney test). Urinary trimethylamine N-oxide (TMAO), arabitol and spermidine showed higher levels in the CVrisk group compared with a control group; while glutamine and pantothenate showed lower levels. The same trend was found for plasma TMAO and glutamine. Plasma choline, acetylcholine and valine were also decreased in CVrisk group, while pyruvate was found increased. In the secretome of HA-VSMCs, TMAO, pantothenate, glycerophosphocholine, glutathion, spermidine and acetylcholine increased after pro-atherosclerotic stimulus, while secreted glutamine decreased. At intracellular level, TMAO, pantothenate and glycerophosphocholine increased with stimulation. Observed metabolic deregulations pointed to an inflammatory response together with a deregulation of oxidative stress counteraction. Keywords: cardiovascular risk; atherosclerosis; chronic kidney disease; vascular smooth muscle cells; oxidative stress; metabolites; metabolomics; biomarkers es_ES
dc.format application/pdf es_ES
dc.language eng es_ES
dc.rights CC-BY es_ES
dc.rights.uri http://creativecommons.org/licenses/by/4.0/deed.es es_ES
dc.title Metabolic Alterations Identified in Urine, Plasma and Aortic Smooth Muscle Cells Reflect Cardiovascular Risk in Patients with Programmed Coronary Artery Bypass Grafting es_ES
dc.type info:eu-repo/semantics/article es_ES
dc.rights.accessrights info:eu-repo/semantics/openAccess es_ES
dc.identifier.location N/A es_ES


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