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AKT and JUN are differentially activated in mesenchymal stem cells after infection with human and canine oncolytic adenoviruses

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Perisé Barrios, Ana Judith & Rodríguez Milla, Miguel Ángel & Morales Molina, Alvaro & Cejalvo, Teresa & García Castro, Javier .AKT and JUN are differentially activated in mesenchymal stem cells after infection with human and canine oncolytic adenoviruses.

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Perisé Barrios, Ana Judith & Rodríguez Milla, Miguel Ángel & Morales Molina, Alvaro & Cejalvo, Teresa & García Castro, Javier. AKT and JUN are differentially activated in mesenchymal stem cells after infection with human and canine oncolytic adenoviruses.

https://hdl.handle.net/20.500.12080/24065
dc.contributor.author Perisé Barrios, Ana Judith
dc.contributor.author Rodríguez Milla, Miguel Ángel
dc.contributor.author Morales Molina, Alvaro
dc.contributor.author Cejalvo, Teresa
dc.contributor.author García Castro, Javier
dc.date.accessioned 2021-03-01T15:51:37Z
dc.date.available 2021-03-01T15:51:37Z
dc.date.created 2020-05-27
dc.identifier.uri https://hdl.handle.net/20.500.12080/24065
dc.description.abstract There is increasing evidence about the use of oncolytic adenoviruses (Ads) as promising immunotherapy agents. We have previously demonstrated the clinical efficiency of mesenchymal stem cells (MSCs) infected with oncolytic Ads as an antitumoral immunotherapy (called Celyvir) in human and canine patients, using ICOVIR-5 or ICOCAV17 as human and canine oncolytic Ads, respectively. Considering the better clinical outcomes of canine patients, in this study we searched for differences in cellular responses of human and canine MSCs to Ad infection that may help understand the mechanisms leading to higher antitumor immune response. We found that infection of human and canine MSCs with ICOVIR-5 or ICOCAV17 did not activate the NF-¿B pathway or the interferon regulatory factors IRF3 and IRF7. However, we observed differences in the profile of cytokines secretion, as infection of canine MSCs with ICOCAV17 resulted in lower secretion of several cytokines. Moreover, we showed that infection of human MSCs with ICOVIR-5 increased the phosphorylation of a number of proteins, including AKT and c-JUN. Finally, we demonstrated that differences in regulation of AKT and c-JUN in human and canine MSCs by ICOVIR-5 or ICOCAV17 are intrinsic to each virus. Our findings suggest that ICOCAV17 induces a more limited host response in canine MSCs, which may be related to a better clinical outcome. This result opens the possibility to develop new human oncolytic Ads with these specific properties. In addition, this improvement could be imitated by selecting specific human MSC on the basis of a limited host response after Ad infection. es_ES
dc.format application/pdf es_ES
dc.language eng es_ES
dc.rights CC-BY es_ES
dc.rights.uri N/A es_ES
dc.title AKT and JUN are differentially activated in mesenchymal stem cells after infection with human and canine oncolytic adenoviruses es_ES
dc.type info:eu-repo/semantics/article es_ES
dc.rights.accessrights info:eu-repo/semantics/openAccess es_ES
dc.identifier.location N/A es_ES


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